Combined used of long-lasting insectiticidal nets and Bacillus thuringiensis israelensis larviciding, a promising integrated approach against malaria transmission in northern Côte d'Ivoire

Abstract

Abstract Background The recent reduction in malaria burden in Côte d’Ivoire is largely attributable to use of long-lasting insecticidal nets (LLINs). However, this progress is threatened by insecticide resistance and behavioral changes in Anopheles gambiae sensu lato (s.l.) populations and residual malaria transmission, and complementary tools are required. Thus, this study aimed to assess the efficacy of the combined use of LLINs and Bacillus thuringiensis israelensis (Bti), in comparison with LLINs.Methods This study was conducted in the health district of Korhogo, northern Côte d'Ivoire, within two study arms (LLIN + Bti arm and LLIN-only arm) from March 2019 to February 2020. In the LLIN + Bti arm, Anopheles larval breeding sites were treated every fortnight with Bti in addition to the use of LLINs. Mosquito larvae and adults were sampled and identified morphologically to genus and species using standard methods. An. gambiae s.l. complex members were determined using a polymerase chain reaction technique. Plasmodium infection in An. gambiae s.l. and malaria incidence in local people was also assessed.Results Overall, Anopheles spp. larval density was lower in the LLIN + Bti arm 0.61 [95% CI: 0.41–0.81] larva/dipper (l/d)) compared with the LLIN-only arm 3.97 [95% CI: 3.56–4.38] l/d (OR = 6.50; 95% CI: 5.81–7.29; P < 0.001). The overall biting rate of An. gambiae s.l. was 0.59 [95% CI: 0.43–0.75] biting/person/night in the LLIN + Bti arm against 2.97 [95% CI: 2.02–3.93] biting/person/night in LLIN-only arm (P < 0.001). An. gambiae s.l. was dominated by An. gambiae sensu stricto (s.s.) (95.1%, n = 293), followed by An. coluzzii (4.9%; n = 15). The high human-blood index was 80.5% (n = 389) in study area. EIR was 1.36 infected bites/person/year (ib/p/y) in the LLIN + Bti arm against 47.71 ib/p/y in the LLIN-only arm. Malaria incidence dramatically declined from 40.5‰ (n = 765) to 15‰ (n = 292) in LLIN + Bti arm (P < 0.001).Conclusions The combined use of LLINs with Bti significantly reduced the incidence of malaria. The LLINs and Bti duo could be a promising integrated approach for effective vector control of An. gambiae s.l for elimination of malaria.