Suwonella silvatica gen. nov., sp. nov., a new genus of family Neisseriaceae isolated from forest soil in Suwon, South Korea by a soil substrate membrane system

Abstract

Abstract A novel Gram-stain-negative, aerobic, non-motile and rod-shaped bacterial strain designated JH1-16T, was isolated from a forest soil sample in Suwon, Republic of Korea by using a soil substrate membrane system. The strain grew well on Luria-Bertani agar, Mueller-Hinton agar, nutrient agar, R2A agar and tryptic soy broth agar. Strain JH1-16T could grow at temperature of 10–35 °C (optimum, 25–28 °C on R2A, MHA and TSB agar), tolerated pH 9.0 and 2.5% (w/v) NaCl. Phylogenetic analysis based on the sequences of 16S rRNA gene and whole genome indicated that JH1-16T made a distinct cluster with members of the family Neisseriaceae. Strain JH1-16T showed the highest 16S rRNA sequence similarity to Vogesella oryzae L3B39T (93.3%), Crenobacter cavernae K1W11S-7T (93.2%), Vogesella amnigena Npb-02T (93.2%), Crenobacter luteus YIM 78141T (92.9%). The major polar lipid profile comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two unidentified aminolipids, and an unidentified phospholipid. The major respiratory quinone system was ubiquinone Q-8. The main fatty acids were C12:0, C16:0, C12:0-3OH, and summed feature 3. The DNA G + C content of the isolated strain was 63.8 mol% calculated by the whole genome sequence. The average nucleotide identity and digital DNA–DNA hybridization between strain JH1-16T and its reference type strains were below the species threshold values. Based on evidences of polyphasic approach, strain JH1-16T (= KACC 21685T = JCM 35861T) could be proposed as the type strain of a new genus and species in the family Neisseriaceae, for which the name Suwonella silvatica gen. nov. is proposed.