qMaLioffG: A single green fluorescent protein FLIM indicator enabling quantitative imaging of endogenous ATP-Reference-Cited by-同舟云学术

qMaLioffG: A single green fluorescent protein FLIM indicator enabling quantitative imaging of endogenous ATP

Published:2023-09-28 Issue: Volume: Page:
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Author:

Arai Satoshi¹,Itoh Hideki²,Vu Cong Quang¹,Nakayama Mizuho³,Oshima Masanobu³,Morita Atsuya³,Okamoto Kazuko⁴,Okuda Satoru¹,Teranishi Aki¹,Osawa Madori⁵,Tamura Yoshiteru⁵,Nonoyama Shigeaki⁵,Takuma Megumi⁶,Fujie Toshinori⁶,Sarker Satya¹,Sudhaharan Thankiah⁷,Kiya Taketoshi⁸^ORCID,Lane E. Birgitte²,Kitaguchi Tetsuya⁹

Affiliation:

1. WPI Nano Life Science Institute, Kanazawa University

2. Skin Research Institute of Singapore, Agency for Science, Technology and Research

3. Division of Genetics, Cancer Research Institute, Kanazawa University

4. Amphibian Research Center, Hiroshima University

5. Department of Pediatrics, National Defense Medical College

6. School of Life Science and Technology, Tokyo Institute of Technology

7. Research Support Centre, Agency for Science, Technology and Research

8. Division of Life Sciences, Graduate School of Natural Science and Technology, Kanazawa University

9. Tokyo Institute of Technology

Abstract

Abstract The widespread use of fluorescence lifetime imaging microscopy (FLIM) for quantitative imaging is hindered by the limited availability of FLIM-based genetically encoded indicators compatible with conventional 488 nm lasers. Here, we present qMaLioffG, a single green fluorescent protein FLIM indicator that shows fluorescence lifetime changes in ATP concentrations within the physiological range. This allows the quantitative imaging of endogenous ATP to investigate the cellular energy status of different cell types.

Publisher

Research Square Platform LLC

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