USPIO combined with MR to long-term show the stem cells to repair the defect of articular cartilage

Abstract

Abstract Backgroud: Magnetic Resonance Imaging (MRI) is used to monitor the process of repairing articular cartilage defect by transplanting the bone marrow mesenchymal stem cell marked by Ultrafine Superparamagnetic Iron Oxide (USPIO) in the rabbit. Methods The Bone Marrow Stromal Cells (BMSCs) cells are isolated from rabbits injected with USPIO (20 mg/kg). Staining and cck-8 are used to detect the ability to form bone, fat cartilage. In the 4 rabbit groups of the double knee joint cartilage defect, the 3 groups are respectively injected the BMSCs-sodium alginate gel compound marked USPIO, BMSCs-sodium alginate gel compound, pure sodium alginate gel, while the blank group doesn't do anything. The rabbits are assessed by MRI and histological studying. Results Compared with the control group, the BMSCs marked by USPIO are the same in cell morphology. The proliferative and differentiation ability of BMSCs in rabbits is not affected by USPIO with a concentration of 20mg/kg. We find that the cartilage defects are gradually repaired in the two cell transplantation groups, and it is slowly repaired by BMSCs transplanting for the treatment of rabbit knee cartilage defects in the other groups. The labeled BMSCs could be used to treat the rabbit knee cartilage defect but could be indicated by MRI. Conclusion The experimental results show that the BMSCs marked by USPIO are the same in cell morphology compared with the control group.