First insight into the genomes of the Pulmonaria officinalis group (Boraginaceae) provided by repeatome analysis and comparative karyotyping

Author:

Kobrlová Lucie1,Čížková Jana2,Zoulová Veronika2,Vejvodová Kateřina3,Hřibová Eva2

Affiliation:

1. Palacký University

2. Institute of Experimental Botany

3. University of South Bohemia

Abstract

Abstract Background The genus Pulmonaria (Boraginaceae) represents a taxonomically complex group of species in which morphological similarity contrasts with striking karyological variability. The presence of different numbers of chromosomes in the diploid state suggests multiple hybridization/polyploidization events followed by chromosome rearrangements (dysploidy). Unfortunately, the phylogenetic relationships and evolution of the genome, have not yet been elucidated. Our study focused on the P. officinalis group, the most widespread species complex, which includes two morphologically similar species that differ in chromosome number, i.e. P. obscura (2n = 14) and P. officinalis (2n = 16). Ornamental cultivars, morphologically similar to P. officinalis (garden escapes), whose origin is unclear, were also studied. Here, we present a pilot study on genome size and repeatome dynamics of these closely related species in order to gain new information on their genome and chromosome structure. Results Flow cytometry confirmed a significant difference in genome size between P. obscura and P. officinalis, corresponding to the number of chromosomes. Genome-wide repeatome analysis performed on partial Illumina sequencing data showed that retrotransposons were the most abundant repeat type, with a higher proportion of Ty3/Gypsy elements, mainly represented by the Tekay lineage. Comparative analysis revealed no species-specific retrotransposons or striking differences in their copy number between the species. A new set of chromosome-specific cytogenetic landmarks, represented by satellite DNAs, showed that the chromosome structure in P. officinalis was more variable compared to that of P. obscura. Comparative karyotyping strongly supported the hybrid origin of putative hybrids with 2n = 15 collected from a mixed population of both species and outlined the origin of ornamental garden escapes, confirming their derivation from the P. officinalis complex. Conclusions Large-scale genome size analysis and repeatome characterization of the two morphologically similar species of the P. officinalis group improved our knowledge of the genome dynamics and differences in the karyotype structure. A new set of chromosome-specific cytogenetic landmarks was identified and used to reveal the origin of putative hybrids and ornamental cultivars morphologically similar to P. officinalis.

Publisher

Research Square Platform LLC

Reference117 articles.

1. The borage family (Boraginaceae s.s.): a revised infrafamilial classification based on new phylogenetic evidence, with emphasis on the placement of some enigmatic genera;Chacón J;Taxon,2016

2. Dysploidie bei Pulmonaria;Merxmüller H;Rev Roum Biol-Botanique,1969

3. Karyo-systematische Untersuchungen an der Gattung Pulmonaria (Boraginaceae). Chromosomen-Zahlen, Karyotyp-Analysen und allgemeine Hinweise auf die Entwicklungsgeschichte;Sauer W;Bibliot Bot,1975

4. A phylogenetic model for North-Eurasiatic Tubifloras on the base of comparative morphology, zytogenetics and ecology;Sauer W;Acta Bot Yunnanica,1986

5. Cytotaxonomy of the genus Pulmonaria L. in Slovakia;Májovský J;Bol Soc Brot Ser 2,1980

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3