Study on non-targeted metabolomics of intestinal tract of DEV- infected ducks

Abstract

Abstract Introduction Duck enteritis virus (DEV) mainly causes infectious diseases characterized by intestinal hemorrhage, inflammation and parenchymal organ degeneration in ducks and other poultry. However, its mechanism of intestinal damage in ducks is not well understood. Metabolomics can provide an in-depth understanding of the full complexity of the disease. Methods In this study, 24 clinically healthy green-shell ducks (weight 1.5kg ± 20g) were randomly divided them into 2 groups (experimental group of 18 rats and control group of 6 rats). The experimental group was intramuscularly injected with 0.2 mL of DEV virus liquid (TCID50 is 3.16×10 -9 /0.1 mL), and the control group was injected with 0.2 sterilized normal saline. mL; at 66 h, 90 h and 114 h after injection (fasting for 12 h before killing), tissue samples from the duodenum and ileum were collected and analyzed by LC-MS. Results Compared with the control group, in the positive/negative mode, the metabolic pathways involved in the differential metabolites in the duodenum of DEV-infected ducks at 66h, 90h and 114h were basically the same; the metabolic pathways related to inflammatory response and immune response were tryptophan Acid metabolism, cysteine-methionine metabolism, histidine metabolism and other amino acid metabolism and fat metabolism. Among them, the metabolic pathways with more differential metabolites are amino acid biosynthesis, cysteine and methionine metabolism, tryptophan metabolism, Unsaturated fatty acid biosynthesis and purine metabolism, the metabolic pathways with more enrichment factors were IgA-related intestinal immune network pathway and lysosome pathway. Compared with the control group, there were 16 differential metabolites in the ileum tissue of DEV-infected ducks at 66 hours of infection, 52 at 90 hours of infection, and 40 at 14 hours of infection with TD114, among which the metabolic pathways with more enriched differential metabolites were Pyrimidine metabolism, tyrosine metabolism, phenylalanine metabolism and tryptophan biosynthesis, the metabolic pathways with more enrichment factors are mTOR signaling pathway, ferroptosis pathway, tryptophan metabolism pathway and caffeine metabolism pathway Conclusion The differential metabolites of DEV-infected ducks are enriched in metabolic pathways such as tryptophan metabolism, amino acid biosynthesis, cysteine-methionine metabolism, unsaturated fatty acid biosynthesis and purine metabolism, among which tryptophan metabolism pathway The enrichment is the most obvious and can be used as a follow-up study;