Is proanthocyanidin associated to vitamin E or palm oil able to protect enamel against initial erosion in vitro?

Abstract

Abstract Objectives This in vitro study evaluated the effect of Proanthocyanidin, Palm Oil and Vitamin E against initial erosion. Materials and Methods Bovine enamel blocks (n = 140) were divided into 14 groups: PC_SnCl2/NaF/Am-F-containing solution (positive control); NC_deionized water (negative control); PO_palm oil; P6.5_6.5% proanthocyanidin; P2_2% proanthocyanidin; VE_Vitamin E; POP6.5_palm oil + 6.5% proanthocyanidin; P6.5PO_6.5% proanthocyanidin + palm oil; POP2_palm oil + 2% proanthocyanidin; P2PO_2% proanthocyanidin + palm oil; VEP6.5_Vitamin E + 6.5% proanthocyanidin; P6.5VE_6.5% proanthocyanidin + Vitamin E; VEP2_Vitamin E + 2% proanthocyanidin; P2VE_2% proanthocyanidin + Vitamin E. The acquired enamel pellicle (AEP) was previously formed in situ for 30 min. The specimens were treated in vitro with the solutions (500 µl, 30s). Then, the blocks were maintained for an additional hour in oral cavity to develop the modified AEP. The blocks were immersed in 0.5% citric acid (pH 2.5) during 30s. The response variable was the percentage of surface hardness loss. Data were analyzed by one-way ANOVA and Fisher's Test (p < 0.05). Results P6.5VE was the only group to promote protection similar to PC. PO, P2, POP6.5, P2PO, P6.5VE and P2VE exhibited %SHL similar to both PC and NC groups. (p < 0.05). P6.5, VE, P6.5PO, POP2, VEP6.5 and VEP2 were different to PC and similar to NC. Conclusion It is concluded that proanthocyanidin associated to vitamin E was able to protect enamel against in vitro initial erosive challenge. Clinical Relevance: This study suggests the potential for a cost-effective anti-erosion product. Further investigations across diverse experimental models are warranted to validate these promising outcomes.