Determination of Performance Characteristics (eLOD50) for Verification of Qualitative Food Microbiological Measurement Methods Based on EN ISO 16140-3:2021-Reference-Cited by-同舟云学术

Determination of Performance Characteristics (eLOD50) for Verification of Qualitative Food Microbiological Measurement Methods Based on EN ISO 16140-3:2021

Published:2022-11-10 Issue: Volume: Page:
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Author:

Khezri Mohammad¹,Khanzadi Saeid¹,Hashemi Mohammad²

Affiliation:

1. Ferdowsi University of Mashhad

2. Mashhad University of Medical sciences

Abstract

Abstract Verification of methods for the microbiological examination of foods is vital according to good laboratory practices (GLP) and ISO/IEC 17025. Microbiological reference methods increase the reliability of food quality control laboratory results. This study aimed to verify three qualitative reference methods by calculating the limit of detection at 50% (eLOD50) according to ISO 16140-3 and to address the related challenges for food microbiology laboratories. We determined the eLOD50 for detecting Escherichia coli, Staphylococcus aureus, and Salmonella spp. in different food items. For method implementation verification, the selected food items were fresh cheese Salmonella spp. and pasteurized milk for E. coli and S. aureus. For food item verification, the eLOD50 values of seven other food items (hamburger, black pepper, soybean powder, ultra-high temperature milk, minced meat, whole liquid pasteurized egg, and cake) were also assessed. The eLOD50 values were 0.5 and 1.9 CFU/test portion of pasteurized milk for E. coli and S. aureus, respectively, and 7.5 CFU/test portion in fresh cheese for Salmonella species. All calculated eLOD50 values were compared with acceptance limits. The calculated eLOD50 values of the qualitative methods complied with the acceptance criteria and were approved for all the selected food items.

Publisher

Research Square Platform LLC

Reference31 articles.

1. IEC I. ISO/IEC 17025: 2017. General requirements for the competence of testing and calibration laboratories. International Organization for Standardization and International Electrotechnical Commission. 2017.

2. Validación de métodos analíticos, fisicoquímicos y microbiológicos;Arriola L;Ministerio de Salud publica de Guatemala Guatemala,2012

3. Hitchins AD. 14 Statistical approaches to develop and validate microbiological analytical methods. Mathematical and Statistical Methods in Food Science and Technology. 2014:231 – 48.

4. Velasco Rodríguez EA, Prada Rodríguez TA, Fonseca Almanzar AM. Protocolo de validación para el método horizontal de detección de salmonella spp en muestras de alimento para consumo animal de un laboratorio de microbiología de una empresa avícola de Bucaramanga en el año 2017. 2017.

5. ISO E. 16140 (2003) Microbiology of food and animal feeding stuff. Protocol for the validation of alternative methods. European Committee for Standardization. International Organization for Standardization, Geneva.