Comparative analysis on genome-wide of NF-Y transcription factors between ‘Pinot Noir’ and Vitis amurensis

Abstract

Abstract Nuclear factor Y (NF-Y) play a crucial role in the response of plants to abiotic stresses. However, there is a lack of research on the comparative analysis of bioinformatics and RT-qPCR of NF-Y transcription factors in ‘Pinot Noir’ and Vitis amurensis. In this study, a total of 27 and 26 NF-Y transcription factors were identified in ‘Pinot Noir’ and Vitis amurensis, respectively，and were divided into 3 subgroups. The subcellular localization prediction revealed that they were mainly located in the nucleus. Conserved motif analysis showed that five motifs from the NF-YB transcription factors exhibited higher conservation than the NF-YA and NF-YC transcription factors. The analysis of NF-Y transcription factor promoters showed that there were various stress-related elements such as drought, anaerobic induction, and ABA, etc. RT-qPCR analysis of ‘Pinot Noir’ and ‘Zuoyouhong’ plantlets demonstrated that the expression levels of VaNF-YA6, VaNF-YB5, VvNF-YA3, VvNF-YA5, and VvNF-YC2, etc. significantly upregulated under 400 mmol·L-1 NaCl and 10% PEG treatments. Furthermore, subcellular localization using tobacco leaves analysis showed that the VaNF-YA6-GFP fusion protein was primarily located in the nucleus. The overexpression of VaNF-YA6 in Arabidopsis can significantly enhance Arabidopsis tolerance to salt and drought stresses by activating antioxidant enzyme activity.