Bio-stimulated synthesis of Zinc oxide Nanoparticles from Andrographis echioides (L.) Nees., characterization and its anti diabetic activity

Abstract

Abstract Green synthesis is based on plant compounds' ability to reduce metal ions and stabilise them into nanoparticles, and it is gaining a lot of attention in recent years. It has been shown to be a reliable and alternate method for the development of novel Nanoparticles. Andrographis echioides (L.) Nees is a used to synthesize zinc oxide nanoparticles. UV-visible spectroscopy, FTIR, Zeta potential, particle size analysis, XRD, SEM, AFM analysis were adopted to characterise the capped ZnONPs. The intense surface Plasmon resonance bands of ZnONPs at 340 nm confirmed the green synthesis of ZnONPs. The various functional groups of A.echioidesinvolved in the synthesis and crystalline nature of ZnO Nanoparticles were identified using FTIR and X-ray diffraction respectively. The average particle size was found to be 4.0 nm, and the zeta potential value was -11 mV. Administration of ZnONPs significantly reduced the blood glucose level and HbA1C when compared with standard drug glibenclamide in STZ- induced in-vivo models. The antidiabetic potential of ZnONPs was confirmed by numerous biochemical parameters like serum lipid profiles, liver and renal functional markers and regeneration β- cells of islets of Langerhans in STZ- induced diabetic rats. Thus, the ZnONPs synthesized from A. echioideswere found to be effective anti-hyperglycemic agent in the management of diabetes.