Autophagy related gene CEP55 is a potential biomarker for osteoarthritis

Abstract

Abstract Objective To screen the target autophagy related genes that may play an important role in the development of osteoarthritis. Methods We analyzed the differentially expressed genes between osteoarthritis samples and control samples in the online dataset GSE46750. We compared these differential genes with autophagy database, and screened out autophagy genes whose expression levels changed significantly in the process of osteoarthritis.We found the Hub gene in these autophagy related genes by constructing a protein interaction network, and analyzed the potentiality of these genes to distinguish patients with osteoarthritis, and constructed the interaction network between these genes and miRNA, further clarifying their potential functions. Results Compared with the control group, the expression of 105 autophagy related genes in Osteoarthritis tissue samples was significantly different. Among these 105 autophagy related genes, 15 Hub genes(AURKA,BIRC5,BUB1,CDC20,CEP55,FOXM1,KIFC1,MAD2L1,MCM2,MCM4,MCM5,RACGAP1,CDCA3,OIP5 and LMNB1) are located at the core of the protein interaction network.The expression level of these Hub genes can distinguish Osteoarthritis tissue samples from control samples, in which CEP55 can be a reliable marker for distinguishing OA from normal individuals,and it also can regulate the expression of miRNA, so CEP55 may be a good target for the treatment of OA by regulating the autophagy level. Conclusion In the development of Osteoarthritis, there is a regulatory network of autophagy related genes. CEP55 is a key gene regulating autophagy process, and it has the potential to interact with miRNA.