Isolation and genetic characterization of novel bovine parechovirus from Japanese black cattle

Abstract

Abstract Novel bovine parechoviruses (Bo_ParVs) were isolated from the feces of Japanese black cattle. Phylogenetic analysis revealed that the novel Bo_ParVs formed an independent cluster, exhibiting 72.2%-75.6% nucleotide and 85.7%-87.0% amino acid sequence identities with previous Bo_ParVs, suggesting that these strains represent a new genotype. Bo_ParVs, including the novel Bo_ParVs, shared sequence similarity with each other in the 3' untranslated region (3'UTR) and exhibited low sequence identities (< 38.9%) to other parechoviruses. However, secondary structure prediction of the 3'UTR revealed that Bo_ParVs shared conserved motifs with the Parechovirus-B and E viruses, suggesting that they have evolved in the same manner.