Knockdown of hsa_circ_0000530 Inhibits Osteosarcoma Progression by Regulating miR-198 and CDK6

Abstract

Abstract Background Circular RNA was reported tightly associated with the incidence and progress of tumor, including osteosarcoma (OS). Circ_0000530, a newly discovered circular RNA, the expression and function were not investigated in OS until now. Here, we aimed to explore it. Materials and Methods By adopting real-time quantitative polymerase chain reaction (qRT-PCR), the level of circ_0000530, miR-198, and cyclin-dependent kinase 6 (CDK6) was identified. Moreover, flow cytometry and transwell assays were adopted for investigating cell cycle distribution, cell migration as well as invasion abilities. Furthermore, using dual-luciferase reporter gene experiments, the correlation between miR-198 and circ_0000530 or CDK6 was revealed. CDK6 protein levels were detected by western blotting (WB). In addition, the impact of circ_0000530 silencing on the growth of OS tumor in vivo was also validated. Results Circ_0000530 levels were raised in OS tissues and cells. Besides, it was shown that knockdown of circ_0000530 could hinder the proliferation, migration as well as invasion of OS cells, also inducing cell cycle arrest. Circ_0000530 could be adsorbed to miR-198, and miR-198 inhibitor was capable of reversing the inhibitory impact of silenced circ_0000530 on OS progression. Silencing of circ_0000530 could hinder CDK6 expression whereas miR-198 inhibitor could be used to recover the impact. Apart from that, circ_0000530 knockdown could reduce OS tumor growth through the regulation of CDK6 expression based on miR-198 in vivo. Conclusion Down-regulation of circ-0000530 inhibits the development of osteosarcoma through the regulation of miR-198 and CDK6, providing new ideas for diagnosing and treating osteosarcoma.