Establishment of the Rapid Propagation System of Actinidia arguta

Abstract

Abstract The effects of different growth regulator types and ratios on the induction of adventitious shoots and adventitious roots were investigated using Actinidia arguta leaves as materials. 'Purple Sadova' was found to have strong germination and the highest germination rate on induction media with different PGRs concentrations. In this experiment, the best medium for the induction of adventitious shoots was MS + ZT 3.0 mg·L− 1 + NAA 0.1 mg·L− 1, with a germination rate of 36.67%. The most suitable site for the induction of adventitious shoots was the base of the leaves, with a germination rate of 80.00%. The longer the dark culture time was, the more suitable for adventitious bud germination, and the germination rate of adventitious buds reached 80.00% after 30 d of dark culture. The best proliferation medium for adventitious buds was MS + 6-BA 1.0 mg·L− 1+NAA 0.3 mg·L− 1, with a proliferation coefficient of 3.57. The proliferated buds had strong growth potential, large leaves, green color and high buds. The best rooting medium was 1/2MS + IBA 0.7 mg·L− 1, with 100% rooting rate, average root length of 2.81 cm and average root number of 14.5. In conclusion, this study explored an effective method to improve the quantity and quality of reproduction of this A. arguta, enriched the fast breeding system of A. arguta, and provided a theoretical basis for the factory breeding of all-red type A. arguta seedlings.