A novel histidine functionalized chitosan nanoformulation: Synthesis, characterization and its bioactivity in tomato plant

Author:

Meena Mahendra1,Saharan Vinod2,Meena K. K.1,Singh Balraj1,Pilania Shalini2,Gupta N. K.1,Pal Ajay3,Garhwal O. P.1,Sharma Y. K.1,Singh Uadal1,Bagri Rajesh1,Sharma M. K.1,Sharma Rachna4,Jakhar B. L.1,Chandel Piyush2,Prajapati Damyanti2,Mondal Kinjal2,Mahala Mital2,Bairwa D. K.1,Meena Madhu Bai2

Affiliation:

1. SKN Agriculture University

2. Maharana Pratap University of Agriculture and Technology

3. Chaudhary Charan Singh Haryana Agricultural University

4. Dr. B. R. Ambedkar National Institute of Technology Jalandhar

Abstract

Abstract The use of novel active ingredients for the functional modification of chitosan nanoformulations has attracted global attention. In this study, chitosan has been functionalized via histidine to craft novel chitosan-histidine nanoformulation (C-H NF) via the ionic gelation method. C-H NF exhibited elite physico-biochemical properties, influencing physiological and biochemical dynamics in tomato crops. Elite properties include homogenous-sized nanoparticles, lower PDI, viscosity, higher zeta potential, nanoparticle concentration/ml, conductivity, encapsulation efficiency, and yield. FTIR spectroscopy revealed histidine interaction with C-H NF, while SEM and TEM exposed its porous structure. Application of C-H NF to tomato seedling and potted plants via seed treatment and foliar spray positively impacts growth parameters, antioxidant-defense enzyme activities, reactive oxygen species (ROS) content, and chlorophyll and nitrogen content. We claim that the histidine-functionalized chitosan nanoformulation enhances physico-biochemical properties, highlighting its potential to elevate biochemical and physiological processes of tomato plant.

Publisher

Research Square Platform LLC

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