Investigation of Virulence and Antibiotic Resistance Genes of E. Coli Strains Isolated from Duhok Hospital by Bacteriological and Molecular Methods

Abstract

Abstract Background The existence of different subsets related to virulence-associated genes in specific path types that are lacking in commensal isolates explains the wide range of pathogenic characteristics and clinical symptoms induced through E. coli path Amis : Using PCR-method to investigate E. coli phylogenetic analysis. Detecting the prevalence related to virulence and antibiotic resistance genes in E. coli, as well as their distribution within phylogenetic groupings. Methods 365 E. coli UTI infection isolates were collected from clinical cases from three major hospitals in Duhok -Iraq.Two hundred and five (56.16%) isolated from male patients and 165 (43.83%) from female patients. One hundred isolates were extracted with a commercial kit, with an average of the concentration of genomic DNA extracted using the commercial kit at 115.25ng/µl with a purity of 1.8. Results to confirm that the genome of all isolates is E. coli, all strains with a molecular weight of approximately 657 bp were successfully amplified producing a single band of uidA as the species-specific gene. The results revealed that Pai and hyl genes related to virulence and antibiotic resistance were absent from any of the tested markers in 10 (28%) of these isolates. As a marker for the presence of a pathogenicity island, the Pai gene is the most dominant marker among all other virulence markers with 75%, followed by the hyl gene with 69 percent. Conclusion There may be a spill of information for the resistance circumstance exterior the clinic environment, particularly for the predominance of multiresistant microbes in solid individuals