High-fat Diet Enhances the Growth of Renal Cell Carcinoma and Alters Immune Cells in Spleen, Kidney and Tumor

Abstract

AbstractRenal cell carcinoma (RCC) is strongly associated with abnormal or excessive fat deposition in the body, whose processes include persistent adipose inflammation and other disturbances with the development and function of immune cells. Researchers have recently become more and more interested in understanding how high-fat diet (HFD) affects the development and course of RCC by causing immunological dysfunction. The changes of immune cell groups in RCC, particularly those in normal kidneys and tumors, are, nevertheless, still poorly understood. Consequently, we explore the effect of HFD on the changes of immune cell groups in spleens, normal kidneys and tumors, mainly revealing the changes of T cells, B cells and NK cells, and further preliminarily exploring the changes of NK cell phenotype. Our findings demonstrate that: 1) HFD speeds up the growth of ACHN tumors; 2) HFD increases the frequency of CD45+live cell, T cell and cNK in spleen, increases the frequency of T cell, NK cell and cNK in normal kidney, as well as increases the frequency of CD45+live cell, NK cell and cNK in tumor;3) HFD decreases the frequency of B cell, NK cell and ILC1 in spleen, decreases the frequency of CD45+live cell, B cell, and ILC1 in normal kidney, as well as decreases the frequency of T cell, B cell and ILC1 in tumor. These data will open up new avenues for immunotherapy in individuals with obese renal cell carcinoma.