High intestinal vascular permeability in a murine model for Hirschsprung’s disease; implications for postoperative Hirschsprung-associated enterocolitis

Author:

Suda Kazuto1,Yamada Shunsuke1,Miyahara Katsumi1,Fujiwara Naho1,Kosaka Seitaro1,Abe Kumpei1,Seo Shogo1,Nakamura Shinji2,Lane Geoffrey J.1,Yamataka Atsuyuki1

Affiliation:

1. Juntendo University School of Medicine

2. Juntendo University Graduate School of Medicine

Abstract

Abstract Purpose Intestinal vascular permeability (VP) in a murine model for Hirschsprung’s disease (HD) and postoperative Hirschsprung-associated enterocolitis (HAEC) were investigated. Methods Intestinal VP was determined using a Miles assay using 1% Evans blue injected into a superficial temporal vein of newborn endothelin receptor-B KO HD model (KO) and syngeneic wild-type (WT) mice (n=5, respectively). Extravasated Evans blue in normoganglionic ileum (Ng-I), normoganglionic proximal colon (Ng-PC) and aganglionic distal colon (Ag-DC) was quantified by absorbance at 620nm. Quantitative polymerase chain reaction (qPCR) for Vascular Endothelial Growth Factor A (VEGF-A), VEGF-B, CDH5, SELE and CD31, and immunofluorescence for CD31 were performed. Results VP was significantly higher in Ng-I, Ng-PC, and Ag-DC from KO than WT (p<0.01, p<0.05, and p<0.05, respectively). qPCR demonstrated upregulated VEGF-A in Ng-I and Ag-DC, VEGF-B in Ng-I, and SELE in Ng-I and Ng-PC (p<0.05, p<0.05, p<0.05, p<0.01 and p<0.05, respectively), and downregulated CDH5 in Ng-I and Ng-PC from KO (p<0.05, respectively). Expression of CD31 mRNA in Ng-I and Ag-DC from KO was significantly higher on qPCR (p<0.05) but differences on immunofluorescence were not significant. Conclusions VP may be etiologic for postoperative HAEC throughout the intestinal tract even after excision of aganglionic bowel.

Publisher

Research Square Platform LLC

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3