Affiliation:
1. Guangxi University
2. Guangxi Academy of Agricultural Sciences
Abstract
Abstract
Plants of the genus Vanda exhibit a diverse array of leaf shapes, with the number of flowers varying from few to many, and a rich palette of colors. These characteristics render them highly valuable for ornamental purposes as tropical orchids. This study employed iPBS molecular markers to analyze the genetic diversity and phylogenetic relationships of 36 species of Vanda and 2 species of Aerides. In addition, DNA fingerprint maps were constructed. All these works will provide a reference for the identification, variety conservation, and hybrid breeding of species in Vanda. Out of 83 iPBS primers, six were selected that produced distinct amplification bands, exhibited high polymorphism, and demonstrated good repeatability. These primers were used for PCR amplification of genomic DNA from 38 samples, resulting in a total of 210 bands. Among these, all 210 bands were polymorphic, achieving a polymorphism rate of 100%. Using the POPGENE 32 software, the mean observed number of alleles (Na) for 38 samples was calculated to be 1.9936, the mean effective number of alleles (Ne) was 1.4243, the mean Nei’s genetic diversity index (He) was 0.2778, and the mean Shannon’s information diversity index (I) was 0.4404. These results indicate a rich genetic diversity among the 38 samples. The genetic similarity coefficients among the samples, calculated using NTSYS-pc 2.1 software, ranged from 0.0345 to 0.6667. Based on these coefficients, UPGMA clustering was performed, and at a similarity coefficient of 0.31, the 38 samples were classified into 7 major groups.
Publisher
Research Square Platform LLC
Reference41 articles.
1. Mobile genomic element diversity in world collection of safflower (Carthamus tinctorius L.) panel using iPBS-retrotransposon markers;Ali F;PLoS ONE,2019
2. Dyakia, a new genus from Borneo;Christenson EA;Orchid Dig,1986
3. An infrageneric classification of Holcoglossum Schltr. with a key to the genera of the Aerides-Vanda alliance;Christenson EA;Notes from the Royal Botanic Garden, Edinburgh,1987
4. Genetic diversity analysis and fingerprinting construction of Dendrobium germplasm resources by iPBS marker;Cui XQ;Chinese Journal of Tropical Crops,2021
5. Ethnobotanical studies on orchids of Niyamgiri hill ranges, Orissa, India;Dash PK;Ethnobot. Leaflets,2008