Activation of PKC affects the ventricular restitution properties and arrhythmogenesis through L-type Ca+ current

Abstract

Abstract Objective To investigate the role of protein kinase C (PKC) in action potential duration (APD) restitution and ventricular tachyarrhythmias (VA). Methods and results Rabbits hearts were isolated and prepared for Langendorff perfusion technique. The S1-S2 method and dynamic S1 pacing protocol were performed to construct APD restitution and to induce APD alternans or VA, respectively, at 10 sites throughout the ventricular chamber. Administration of PMA (100 nM) (n = 15) greatly steepened the restitution curves (Smax>1) (P < 0.01) at each site compared to the control group (n = 15). Furthermore, treatment with PMA also induced larger spatial dispersions of Smax (P < 0.05) and decreased the thresholds of the VA and APD alternans (P < 0.01). However, perfused with the PKC inhibitor, BIM (500 nM) (n = 10), reversibly flattened the APD restitution curves at each site (Smax< 1), decreased the spatial dispersions of Smax, and increased the thresholds of APD alternans and VA. According to the results of patch-clamp, peak amplitude of L-type Ca2+ current was significantly increased by addition of PMA compared with CTL group (P < 0.05). Antagonize this current with verapamil (n = 10) can fully inhibited the PMA induced increasing of Smax and inducibility of VA and alternans. Conclusion PKC activation increased the dispersion of APD restitution and thus led to occurrence of VA, which possibly related to the increased Ca2+ influx.