LncRNA AP000842.3 Triggers the Malignant Progression of Prostate Cancer by Regulating Cuproptosis Related Axis

Abstract

Abstract Background LncRNAs and miRNAs have significant regulatory roles in prostate cancer (PRAD) through the ceRNA network. Cuproptosis is a unique form of programmed cell death that is involved in various signaling pathways and biological processes related to tumor development. NFAT5, a transcription factor that activates T cells, has been implicated in cuproptosis. In this study, we aimed to determine the mechanism by which NFAT5 is involved in the regulation of ceRNA networks in prostate cancer. Methods and results The ceRNA network related to the survival of PRAD related genes was constructed by bioinformatics. Dual-luciferase reporter assay, CCK-8 and other experiments were used to prove the conclusion. The regulatory network of ceRNA was constructed by the differentially expressed lncRNA and miRNA and the mRNA after cuproptosis clustering analysis, and it was found that miR-206/NFAT5 may be an important ceRNA axis in the regulation of PRAD. We found that changes in AP000842.3 and miR-206 expression may affect PRAD proliferation through regulation of NFAT5. Mechanistically, AP000842.3 acts as the ceRNA of miR-206 to regulate the expression of NFAT5. The effects of lncRNA AP000842.3 on the malignant progression of PRAD and NFAT5 were partly dependent on miR-206. Conclusions In conclusion, we successfully filtered out that lncRNA AP000842.3, as a ceRNA of miR-206, is involved in regulating the level of cuprotosis-associated transcription factor NFAT5 in PRAD.