Abstract
Wounds provide a suitable environment for microbial attacks and infections, which are among the most significant obstacles to wound healing. Pseudomonas aeruginosa is one of the most common wound infection agents, known for its high antibiotic resistance. The use of herbal antimicrobial agents in traditional methods of treatment poses certain challenges. Nano-liposomes are considered a novel strategy to overcome these challenges. Nano-liposomes were loaded with a combination of thin-layer hydration and ultrasonic waves using lecithin, cholesterol, pistacia atlantica fruit and gum essential oil, and Camellia sinensis (green tea) extract in an inactive form. The physicochemical properties of NLPACS, including size, zeta potential, loading capacity, and drug release profile, were evaluated. The antibacterial property was assessed using microdilution, and the healing property of NLPACS in the form of a wound ointment was evaluated on infected incised wounds in Wistar rats. The research results indicated that NLPACS, with a capsule efficiency of 81%, a size of 109 nm, a zeta potential of -7.23 mV, and controlled release at 37 degrees Celsius, exhibited an acceptable structure. Its MIC and MBC were 156 and 312.5 µg.mL, respectively. The use of NLPACS in ointment form on infected wounds in Wistar rats confirmed its healing and antibacterial properties. The results showed that NLPACS has suitable physicochemical, antimicrobial, and healing properties, making it a potential drug for the healing of incised wounds contaminated with Pseudomonas aeruginosa.