Exploration of the Mechanism of Treating Retinal Vein Occlusion with Channeling Pulse and Increasing Vision Decoction Based on Network Pharmacology and Molecular Docking

Abstract

Abstract Objective To explore the pharmacological mechanism of channeling pulse and increasing vision decoction (CPIVD) in the treatment of retinal vein occlusion (RVO) by using the method of network pharmacology and Molecular Docking. Method Active ingredients and effective targets of CPIVD were collected by using TCMSP database, the related targets of RVO from GeneCards and DisGeNET databases, the cross targets from VENNY diagram, the medicine-composition-target network mutual-aid map from Cytoscape, Searching for genes by retrieving the input proteinthe in Uniprot database, PPI analysis in STRING database and GO and KEGG enrichment analysis from DAVID database. Molecular docking of the main active components and key targets of CPIVD was verified by AutoDock software. Result We screened 24 active components and 210 effective targets from CPIVD, 181 gene targets from RVO and 39 cross targets. PPI network analysis shows the core targets of RVO. GO enrichment analysis had 66 related items, and KEGG enrichment analysis obtained 52 signaling pathways, among which HIF-1, TNF, PI3K-Akt and other signaling pathways were significantly enriched. Molecular docking results showed that the binding energy between quercetin and kaempferol and the five target proteins, including IL6, TNF, IL1B, AKT1 and VEGFA, were all less than-5.0kcal / mol, suggesting good binding activity. Conclusion CPIVD realizes the therapeutic effect on RVO through multi-target, multi-pathway and multi-pathway and predicts the underlying therapeutic mechanisms. It provides theoretical value for further exploring the treatment of RVO with CPIVD.