Electrospun Pullulan-based Nanofibers to Prepare Modified Electrodes for Electrochemiluminescence Detection of Azithromycin

Abstract

Abstract In this paper, a bio-based carbon nanofibers material was prepared by electrospinning, and carbon fiber was further prepared with pullulan as the carbon source and nitrogen source, and a new type of string-shaped bio-based carbon nanofiber was prepared. Using scanning electron microscopy, EDS Energy Dispersive Spectrometer, XPS X-ray photoelectron spectroscopy and XRD X-ray Diffraction to detect and analyze the morphology structure and elements of the material, the above characterization results show that the pullulan-based nanofibers have crossover Type 3D fiber structure. The composite material elements are mainly composed of C elementsand O elements. The pullulan-based carbon nanofiber material can be used for the Electrochemiluminescence detection of azithromycin and exhibits high sensitivity. The detection range of this modified electrode for azithromycin is 1×10− 7-1×10− 4 µmol/L, and the linear correlation coefficient R2 = 0.996. The lower limit of detection (LOD) is 9×10− 8 µmol/L.The standard recovery rate of 10, 50, 70 µmol/L is 99.32–100.31%. Relative standard deviation is less than 5%, This method has good selectivity and high sensitivity, and can be used for the detection of azithromycin.