Complete genomic sequence of a novel botourmiavirus infecting the fungus Phomopsis asparagi

Abstract

Abstract Here, we report a novel ourmia-like mycovirus, named “Phomopsis asparagi magoulivirus 1” (PaMV1), derived from the phytopathogenic fungus Phomopsis asparagi. The complete genome sequence of PaMV1 consisted of a positive-sense single-stranded RNA (+ ssRNA) of 2,639 bp in length with a GC content of 57.13%, containing a single open reading frame (ORF) which encodes a putative RNA-dependent RNA polymerase (RdRp) of 686 amino acids with a molecular mass of 78.57 kDa. RdRp phylogeny indicates that PaMV1 grouped together with Diaporthe gulyae magoulivirus 1 (DgMV1) forming a distinct clade. The results of RdRp sequence homology comparisons and phylogenetic analysis suggested that PaMV1 is a new member of the genus Magoulivirus within the family Botourmiaviridae.