Early diagnosis by mNGS could improve the prognosis for severe leptospirosis patients-Reference-Cited by-同舟云学术

Early diagnosis by mNGS could improve the prognosis for severe leptospirosis patients

Published:2022-09-30 Issue: Volume: Page:
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Author:

Jiang# Xuqin¹,Jiang# Hui²,Xiong# Yingying³,Fang# Ming⁴,Geng Bei⁵,Wu* Huimei⁶,Mei* Xiaodong⁷

Affiliation:

1. Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China

2. Department of Pulmonary and Critical Care Medicine, Wanbei Coal and Power Group General Hospital, Suzhou, China

3. Department of Pediatrics, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China

4. Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Anhui University of Science and Technology, Huainan, China

5. The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China

6. Department of Geriatric Respiratory and Critical Care, Anhui Geriatric Institute, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022.

7. Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China

Abstract

Abstract PurposesTo evaluate the diagnostic efficiency of metagenomics next generation sequencing (mNGS) assay in severe leptospirosis and its role on prognosis. Methods We retrospectively reviewed the medical records of patients with confirmed leptospirosis from Jan. 1, 2019 to Dec. 31, 2021 from three hospitals. Detailed data including socio-demographic data, clinical manifestations, laboratory examination results, CT images etc. were collected and analyzed. Results Six severe cases were included in this study. All were diagnosed by positive mNGS results. Three of the patients were also polymerase chain reaction examined and presented negative results. Five patients were diagnosed by mNGS assay within two or three days and antibiotics were down-graded to ceftriaxone in them. All of them were cured finally. One case, who was diagnosed by mNGS late on day 5, died. Conclusions mNGS is highly efficient in diagnosing leptospirosis and could possibly reduce the mortality rate if used at an early stage.

Funder

National Natural Science Foundation of China

Publisher

Research Square Platform LLC

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