Affiliation:
1. Malatya Turgut Ozal University
2. Bartin University
3. Inonu University
Abstract
Abstract
MOTS-c, a mitochondrial-derived peptide, acts as a systemic hormone and MOTS-c level is inversely correlated with markers of obesity. Obesity is a risk factor for male reproductive physiology and is expressed as an important cause of infertility. In this study, we aimed to determine the effects of MOTS-c, which has been proven in the hypothalamus and testicles, on the actors involved in the reproductive axis. In the study, 80 male Wistar-Albino rats were divided into two main groups, obese and non-obese (n = 40). Rats in the first main group were fed with fatty diet feed and obesity was induced. The second main group was fed with normal diet feed. Each main group was divided into 4 small groups (Control, Sham, 10 and 100 µM MOTS-c). The lateral ventricles of the animals in the treatment groups were infused with 10 and 100 µM MOTS-c (solvent in Sham group) for 14 days. At the end of the experiment, hypothalamic Gonadotropin-Releasing Hormone (GnRH) gene expression level, serum testosterone, Luteinizing hormone (LH) and Follicle stimulating hormone (FSH) levels were determined. MOTS-c infusion caused an increase in GnRH mRNA, protein expression levels and serum testosterone, LH and FSH levels in obese and non-obese rats (p < 0.05). MOTS-c administration more significantly upregulated hormone levels in non-obese rats (p < 0.05). Our results reveal that MOTS-c plays a role in the central regulation of reproductive behavior, as well as causes increased LH, FSH and testosterone release. MOTS-c may emerge as a novel regulator for the prevention of obesity-induced infertility.
Publisher
Research Square Platform LLC
Reference33 articles.
1. Hypothalamic control of the pituitary-gonadal axis in higher primates: key advances over the last two decades;Plant TM;J Neuroendocrinol,2008
2. Mechanism of pulsatile GnRH release in primates: Unresolved questions;Terasawa E;Mol Cell Endocrinol,2019
3. Pulsatile secretion of LH, FSH, prolactin, oestradiol, and progesterone during the human menstrual cycle;Backström CT;Clin Endocrinol,1982
4. O'Donnell L, Stanton P, de Kretser DM (2017) Endocrinology of the Male Reproductive System and Spermatogenesis. In K. R. Feingold (Eds.), Endotext. MDText.com, Inc. PMID: 25905260 Bookshelf ID: NBK279031
5. The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance;Lee C;Cell metab,2015