Soybean peptide preparation and its protective effect on IPEC-J2 cell injury induced by H2O2

Abstract

Abstract The study aimed to illustrate the protective effect of soybean peptides (SBP) on intestinal porcine epithelial cell (IPEC-J2) damage induced by H2O2. SBP were prepared by protease hydrolysis. Cell experiment included four groups: Control, SBP incubation, H2O2 treatment, SBP + H2O2 groups. This study showed that cell viability was significantly decreased when H2O2 concentration was increased. However, the cells pretreated with SBP (100 µg/mL) for 12 h followed by H2O2 treatment at 1mM for 1 h significantly increased cell viability and antioxidant enzyme activity, decreased cell apoptosis, compared with the single H2O2–induced cells. Moreover, the mRNA abundances of Bax, Caspase-3 and Nrf2 were significantly down-regulated; while the mRNA abundances of ZO-1, claudin-1, occludin, catalase, GSH-Px1, SOD1, HO-1 and NQO-1 were significantly up-regulated in SBP + H2O2 group, compared with the single H2O2 group. This study indicated that SBP pretreatment alleviated H2O2-induced cell oxidative stress and protected intestinal cell integrity.