Evaluation of innate lymphoid cells and long-term cognition in a rat model of experimental sepsis

Author:

Catalão Carlos Henrique R.1,Petronilho Fabricia2,Dal-Pizzol Felipe2,Pillai Anilkumar3,Giridharan Vijayasree V.4,Barichello Tatiana4

Affiliation:

1. Department of Neurosciences and Behavioral Sciences of Ribeirão Preto Medical School, University of São Paulo (USP)

2. Graduate Program in Health Sciences, University of Southern Santa Catarina (UNESC)

3. Pathophysiology of Neuropsychiatric Disorders Program, Faillace Department of Psychiatry and Behavioral Sciences, McGovern Medical School, The University of Texas Health Science Center at Houston

4. Translational Psychiatry Program, Department of Psychiatry and Behavioral Sciences, McGovern Medical School, The University of Texas Health Science Center at Houston (UTHealth)

Abstract

Abstract Background Sepsis is characterized as a critical condition involving life-threatening organ dysfunction stemming from an imbalanced host immune response to infections. Survivors of sepsis face an increased risk of post-hospital discharge mortality along with potential long-term cognitive and functional impairments. Recent research has uncovered the roles of innate lymphoid cells (ILCs) in the borders of the central nervous system (CNS), extending beyond their well-established functions in immune regulation and neuroinflammation. ILCs may play a pivotal role in the pathophysiology of sepsis, influencing immunity, inflammation, and brain barrier homeostasis. Here we investigated the role of subtypes ILC1, ILC2, and ILC3 on experimental sepsis-induced cognitive decline. Methods Adult male Wistar rats were submitted to sepsis by cecal ligation and perforation (CLP) surgery or sham (non-CLP) surgery as the control group. Ten days after sepsis induction, all the rats were submitted to a novel object recognition (NOR) task, a non-spatial, declarative memory known as recognition memory and were euthanized. The presence of ILCs was evaluated in the choroid plexus (CP), meninges, brain, spleen, and gut using multi-channel flow cytometry. Results We observed that Wistar rats recovered from sepsis showed deficits in recognition memory, with no discernible difference in the count of ILCs between the CLP and non-CLP groups. However, we found a significant difference between ILC subtypes in the meninges and CP in the CLP group. Conclusions This finding further reinforces the notion that sepsis survivors commonly experience cognitive deficits post-recovery. Additionally, it implies that the involvement of specific ILCs subtypes may be significant during the acute phase of sepsis pathophysiology, but their impact diminishes after the recovery period.

Publisher

Research Square Platform LLC

Reference46 articles.

1. Napolitano LM. Sepsis 2018: Definitions and Guideline Changes. Surg Infect. 2018;19:117–125.

2. Global, regional, and national sepsis incidence and mortality, 1990–2017: analysis for the Global Burden of Disease Study;Rudd KE;Lancet,2020

3. Risk Factors for Long-term Mortality and Patterns of End-of-Life Care Among Medicare Sepsis Survivors Discharged to Home Health Care;Courtright KR;JAMA Netw Open,2020

4. Sepsis and septic shock;Hotchkiss RS;Nat Rev Dis Primers,2016

5. Enhancing Recovery From Sepsis: A Review;Prescott HC;JAMA,2018

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3