Nature-inspired biomolecular corona-based on poly(caffeic acid) as a low potential and time-stable glucose biosensor

Author:

Kuznowicz Maria1,Jędrzak Artur1,Jesionowski Teofil1

Affiliation:

1. Poznan University of Technology

Abstract

Abstract Herein, we present novel biosensor based on nature-inspired poly(caffeic acid) (PCA) grafted to magnetite (Fe3O4) nanoparticles with glucose oxidase (GOx) from Aspergillus niger via adsorption technique. The biomolecular corona was applied to fabrication of biosensor system with screen printed electrode (SPE). The obtained results indicated an operation of the system at low potential (0.1 V). Then, amperometric measurements were performed to optimize conditions like various pH and temperatures. The SPE/Fe3O4@PCA-GOx biosensor presented a linear range from 0.05 mM to 25.0 mM, with a sensitivity of 1198.0 μA mM−1 cm−2 and a limit of detection 5.23 μM, which was compared to other biosensors presented in the literature. The proposed system was selective towards various interferents (maltose, saccharose, fructose, L-cysteine, uric acid, dopamine and ascorbic acid) and shows high recovery in relation to tests on real samples, up to 10 months of work stability. Moreover, the Fe3O4@PCA-GOx biomolecular corona has been characterized using various techniques such as Fourier transform infrared spectroscopy (FTIR), high-resolution transmission electron microscopy (HRTEM), atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS), and Bradford assay.

Publisher

Research Square Platform LLC

Reference45 articles.

1. M.A touch-actuated glucose sensor fully integrated with microneedle array and reverse iontophoresis for diabetes monitoring;Cheng Y;Biosens Bioelectron,2022

2. Diabetes mellitus: The epidemic of the century;Kharroubi AT;World J Diabetes,2015

3. Glucose biosensors for clinical and personal use;Karyakin AA;Electrochem Commun,2021

4. Portable glucose biosensor based on polynorepinephrine@magnetite nanomaterial integrated with a smartphone analyzer for point-of-care application;Jędrzak A;Bioelectrochemistry,2022

5. Jędrzak, A., Kuznowicz, M., Jesionowski, T., Mobile–assisted diagnostic biosensor for point–of–care glucose detection in real human samples with rapid response and long–live stability, J. Appl. Electrochem. 2023, 1–12 (2023).

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