Identification of hub genes and potential mechanisms that linked hyperbaric oxygen therapy to traumatic injury Using Bioinformatics Analysis

Abstract

Abstract Traumatic brain injury (TBI) is a leading cause of acquired disability worldwide. Although hyperbaric oxygen therapy is a treatment for TBI, the mechanism underlying its function remains unclear. Herein, we comprehensively analyzed the RNA sequencing data from public databases using bioinformatics analyses and identified three hub genes (IL10, MMP9, and PECAM1) associated with hyperbaric oxygen therapy and TBI. CIBERSORT was used to analyze patient data to infer the relative proportions of 22 infiltrating immune cells and to perform Pearson correlation analysis on gene expression and immune cell content levels. The three hub genes were significantly associated with infiltrating immune cells and had a predictive ability for TBI. In addition, the transcription factors of these three hub genes were identified and found to be enriched in immune functions. In conclusion, three hub genes and potentially relevant immune cells and biological processes were identified, which are associated with hyperbaric oxygen therapy and TBI. This will provide new evidence for further research on hyperbaric oxygen therapy and TBI.