Intraperitoneal injection of mesenchymal stem cells-conditioned media (MSCS-CM) treated monocyte can potentially alleviate motor defects in experimental autoimmune encephalomyelitis female mice; an original experimental study

Abstract

Abstract Introduction: Multiple sclerosis (MS) is destructive pathology of myelin in central nervous system (CNS) leading to occurrence of physical complications. Experimental autoimmune encephalomyelitis (EAE) is the laboratory model of MS widely used for CNS-associated inflammatory research. Cell therapy using macrophage M2 (MPM2), as a cell type with anti-inflammatory feature, seems useful for EAE treatment. This study was aimed to investigate the therapeutic anti-inflammatory effects of intraperitoneal (IP) injection of MPM2 cells on alleviation of motor defect in female EAE-affected mice. Materials and Methods: 24 C57/BL6 female mice were divided into EAE (EAE-affected mice), EAE+Dexa (EAE-affected mice receiving dexamethasone), EAE+PBS (EAE-affected mice receiving PBS) and EAE+MP2 (EAE-affected mice receiving MPM2). EAE was induced through application of spinal homogenate of guinea pigs. MPM2 cells were extracted and cultured from bone marrow and injected (106cells/ml) in three days of 10, 13 and 16 post-immunization (p.i). The clinical score (CS), anti-inflammatory cytokines, pro-inflammatory gene expression and histopathological investigations were considered. Data were assessed analyzed using SPSS software (v.19) and p<0.05 was assumed significant level. Results: IP injection of MPM2 in EAE-affected mice can significantly (p<0.05) alleviate CS index, accelerate anti-inflammatory cytokines (IL-4, IL-10), and attenuate pro-inflammatory genes expression (TNF-α, IL-1β). These findings were also approved by histopathological tissue staining. Conclusion: IP injection of MPM2 to the EAE-affected mice can potentially reduce the CNS inflammation, neuronal death and myelin destruction leading to the improvement of animal movement behaviors.