Abstract
NAC gene family exists widely in plants and plays an important role in plant response to stress and growth. At present, NAC gene has been identified in many plants, but it has not been fully identified and analyzed in H. ammondendron. In this study, the number and evolution of NAC gene family were analyzed and identified by bioinformatics methods, and the structure, physical and chemical properties, subcellular localization and promoter cis-acting elements of NAC gene family were further analyzed, q-RTPCR was used to analyze the expression pattern analysis. Finally, a total of 71 NAC genes were identified in H. ammondendron, distributed on 9 chromosomes, most of which were located in the nucleus. According to phylogenetic analysis, NAC proteins of H. ammondendron can be divided into seven subfamilies. Analysis of gene structure and conserved domain indicated that different subfamilies had different Motifs, indicating functional diversity. Through qRT-PCR under drought stress and salt stress, HaNAC49 gene was significantly up-regulated under drought stress and salt stress, indicating that they may be involved in regulating the response of H. ammondendron to abiotic stress. Our results indicate that NAC gene family plays an important role in the resistance of H. ammondendron, and provide an important theoretical basis for further research on the function of NAC gene family and the molecular mechanism of H. ammondendron resistance.