Regulation of glioblastoma proliferation and migration by Nova1

Abstract

Abstract Purpose: The aim of this study was to investigate the regulation of proliferation and migration of glioblastoma (GBM) cells by Nova1. Methods: We used a bioinformatics approach to analyze RNA sequencing data from the cerebral cortex of Nova1 knockout mice and that of wild-type mice to screen differentially expressed genes (DEGs). The GSE69709 dataset was downloaded from the Gene Expression Omnibus (GEO) database, and six samples were selected for analysis. Cdhr1 and Pmfbp1 were screened out among 149 DEGs. GBM cell lines U251, U87 and Mo59j were transfected by Nova1 knockdown lentivirus. After verifying transfection efficiency, CCK8, colony formation, scratch and Transwell migration assays were performed to analyze cell proliferation and migration. Based on brain stereotaxic techniques, GBM C6 cells were microinjected into the striatal region of mice to construct an orthotopic xenograft tumor model. The distribution and expression of Nova1 in the subventricular zone(SVZ) and hippocampal dentate gyrus (DG) were analyzed using the TissueFAXS. Results: Nova1 is mainly distributed in the midbrain and brainstem of mice. Nova1 is highly expressed in GBM tissues. Furthermore, knockdown of Nova1 significantly inhibited GBM cell proliferation and migration. Meanwhile, Nova1 can change GBM cell morphology by remodeling the cytoskeleton. Conclusion: Nova1 plays an important role in the occurrence and development of GBM, may be a potential therapeutic target for GBM.