Variabilities and similarities of adult stem cells derived intestinal organoids originating from different intestinal segments in pig

Abstract

Abstract Background Organoids are in vitro model systems generated from tissues. Organoids express specific physiological functions associated with their original tissue location and they express tissue-segment-specific genes. The aim of this study was to investigate the resemblance of duodenum, ileum (with or without Peyer’s Patches (PP) – PP could be recognized visually), and colon tissue-derived organoids with the tissue of origin. Methods and Results We studied the variability of multiple adjacent sampling sites based on histology and transcriptome profiles. The transcriptome profiles of the in vivo tissues and the derived organoids showed high resemblance for all intestinal segments. Transcriptomic cluster analysis showed that it is important to use tissue-specific genes to show the resemble between tissue and their derived organoids. The transcriptome profiles clearly separated the intestinal segments, and samples of the same segment from adjacent tissue locations showed high transcriptome profile similarity. Ileum samples with and without PP were also separated. Pathway analysis of differentially expressed genes from PP compared with non-PP suggested the importance of several aspects of cell cycle progression regulation, including DNA metabolism, chromatin organization, regulation of mitotic stage progression, and regulation of inflammation. Conclusions Organoids reflect the sampled intestinal segment and that organoids derived from adjacent sampling sites in an intestinal tissue segment showed low variability. The results from the ileum indicate that organoids have potential to study intestinal immune processes.