Determination of Oxidative Stress Responses Induced by Copper Oxide (Cuo) Nanoparticle in Gammarus Pulex

Abstract

Abstract As Copper Oxide (CuO) has a wide range of uses in industry, it is thought to have a wide polluting effect on the environment and aquatic environment. Gammarus pulex was chosen as the model organism in this study, which was carried out with the aim of realizing the effect of CuO mixed into the water environment. In order to carry out the study, CuO was exposed to 0 (control), 10, 20, 40 ppm concentrations for 24, 96 and 120 (elimination) hours. Samples were taken from the experimental environment at the end of 24, 96 and 120 hours. The study was carried out in 3 replicates. The assessment of oxidative stress and antioxidant biomarkers was conducted using ELISA kits obtained from CAYMAN Chemical Company. The parameters analyzed included the activity of SOD and CAT, as well as the levels of TBARS and GSH. The biochemical studies were evaluated using the one-way ANOVA (Duncan 0.05) feature of the SPSS 24.0 package program. With the study data, increases in SOD activities and fluctuations in CAT activity were determined. Decreases in GSH levels and increases in TBARS levels occurred.