Necdin Inhibits the Proliferation of Osteosarcoma Cells and Lung Metastasis by Wnt/β-catenin signaling pathway

Abstract

Abstract The emergence of neoadjuvant chemotherapy has improved the prognosis of patients with osteosarcoma (OS), but metastasis and recurrence have become the difficulty of treatment. Necdin (NDN) exists as a tumor suppressor gene in some tumors, but its role in osteosarcoma is not clear, while c-Myc is a clear oncogene. This study found that the expression of NDN is low expressed and c-Myc is high expressed in osteosarcoma. In vitro overexpression of NDN can suppress the proliferation and promote apoptosis of osteosarcoma cells and reduce its invasiveness; at the same time, NDN can inhibit the expression of c-Myc, MMP2, and MMP13. It has been confirmed in vivo studies that NDN is a tumor suppressor of osteosarcoma which can effectively suppress tumor growth and lung metastasis in xenograft nude mice. Transcriptomic testing found and western blot confirmed that NDN affects the expression of c-Myc, MMP2, and MMP13 genes by affecting key proteins of the Wnt/β-catenin signaling pathway. Our results show that NDN can not only inhibits tumor growth in osteosarcoma, but also inhibits the lung metastasis of osteosarcoma by affecting Wnt/β-catenin signaling pathways.