Affiliation:
1. Iran University of Science and Technology
2. shjavan@iust.ac.ir
Abstract
Abstract
As medical research has progressed, the derivation and development of biological materials such as hydrogels have steadily gained more interest. The biocompatibility and non-toxicity of chitosan make chitosan hydrogels potential carriers for drug delivery. This work aims to develop two multi-reactive, safe and highly swellable bio-hydrogels consisting of chitosan-graft-glycerol (CS-g-gly) and carboxymethyl chitosan-graft-glycerol (CMCS-g-gly), for sustained and controlled drug release, improved bioavailability along with entrapment in nanocarriers, which reduces side effects of vincristine sulphate. CS-g-gly and CMCS-g-gly were successfully prepared and fully characterized using FT-IR, 1HNMR, FE-SEM, AFM, TGA, DLS, and zeta potential techniques. Under various conditions, the prepared hydrogels exhibited a high swelling ratio. Vincristine loaded CS-g-gly (VCR/CS-g-gly) and CMCS-g-gly (VCR/CMCS-g-gly) showed high encapsulation efficiency between 72.28–89.97%, and 56.97–71.91%, respectively. VCR/CS-g-gly showed a sustained release behavior, and the maximum release of VCR from hydrogels reached 82% after 120 h of incubation. MCF-7 and MCF-10 cells were evaluated for cell viability and apoptosis induction. The in-vitro anti-tumor efficacy was investigated using flow cytometry. MTT analysis of hydrogels showed no evidence of significant cytotoxicity in MCF-7 and MCF-10 cells. According to these findings, these hydrogels could effectively deliver drugs to MCF-7 and other breast cancer cells.
Publisher
Research Square Platform LLC
Reference86 articles.
1. Global burden of cancer;Ma X;The Yale journal of biology and medicine,2006
2. Rezaei, T., et al., Folic Acid-Decorated pH-Responsive Nanoniosomes With Enhanced Endocytosis for Breast Cancer Therapy: In Vitro Studies. Frontiers in pharmacology, 2022. 13.
3. Improved brain delivery of vincristine using dextran sulfate complex solid lipid nanoparticles: optimization and in vivo evaluation;Aboutaleb E;Journal of Biomedical Materials Research Part A,2014
4. Assessment of Pediatric Chemotherapy-Induced Peripheral Neuropathy Using a New Patient-Reported Outcome Measure: The P-CIN;Smith EML;Journal of Pediatric Oncology Nursing,2021
5. Paul, M.R. and P.E. Zage, Overview and recent advances in the targeting of medulloblastoma cancer stem cells. Expert Review of Anticancer Therapy, 2021(just-accepted).