Whole genome sequencing provides evidence for Bacillus velezensis SH-1471 as a beneficial rhizosphere bacterium in plants

Abstract

Abstract Bacillus spp. is widely used in agriculture due to its diverse biological activities. We isolated a Bacillus velezensis SH-1471 from the rhizosphere soil of healthy tobacco, which has broad-spectrum antagonistic activity against a variety of plant pathogenic fungi such as Fusarium oxysporum, and can be colonized in the rhizosphere of a variety of plants. This study will further explore its mechanism by combining biological and molecular biology methods. B. velezensis SH-1471 contains a ring chromosome of 4181346 bp with a mean G+C content of 46.18%. We identified 14 homologous genes related to biosynthesis of resistant secondary metabolite, and three clusters encoded potential new antibacterial substances. It also contains a large number of genes from colonizing bacteria and genes related to plant bacterial interactions. It also contains genes related to environmental stress, as well as genes related to drug resistance. In addition, we found that there are many metabolites in the strain that can inhibit the growth of pathogens. Therefore, B. velezensis SH-1471 is a potential biological control strain with important application value. The results of this study will help to further study the mechanism of B. velezensis SH-1471 in biological control of plant diseases and promote its application.