Optical Multiparametric Bio-analyte Detection System: Design, Development and Noise Analysis

Abstract

Abstract In this work, we have designed and developed a multiparametric optical detection system for bio-analyte measurement. Five interlinked optical parameters i.e. transmittance, reflectance, internal scattering, surface scattering and output power are measured simultaneously from transmitted and reflected spot images in MATLAB image processing environment. Also RGB channel information of images was studied to find further non-interlinked parameters for chromatic white light source. Detailed noise analysis of the system was performed to study the effect of individual component noise and finally a simple noise reduction method is presented. The system was tested with non-transparent and semi-transparent sensor samples for glucose sensing application and found to have quite sensitive performance inside a particular range of operation. The system was able to measure both real-time data and fixed time interval data for all the interlinked parameters simultaneously. Such sensitive, reliable and cost-effective instrument with multiparametric measurement feature has immense potential in optical biosensor field.