Interrelationship among substrate utilization, metabolic productions, and housekeeping-related gene expression levels in Mortierella alpina CBS 754.68

Abstract

Abstract Mortierella alpina has been well-known for producing a substantial amount of lipid with considerable amounts of long poly unsaturated fatty acid. A combination of excess carbon with nitrogen-limited substrates supplemented with magnesium oxide nanoparticles (MgONPs) were used to assay their effects on lipid and dry weight biomass of Mortierella alpina CBS 754.68 productions and subsequently, the expressions of 10 possible candidate housekeeping genes during the fermentation time. The results indicated that proteins were completely used up over the early stage of the fermentation and, as a result, DWB steadily declined at the end of the fermentation time, while in medium with MgONPs, Mortierella alpina stayed at the growth phase during the fermentation time. GeNorm and BestKeeper software were used to analyze statistically the data of Reverse transcription polymerase chain reaction (RTqPCR) of 10 possible candidate housekeeping genes. Ultimately, actin was rated as a best housekeeping gene candidate when Mortierella alpina experienced both growth and death phases, on the other hand dihydropteridine reductase and 28s were realized as suitable ones when Mortierella alpina stayed in a growth phase.