Isolation, characterization and In-vitro evaluation of potential probiotic lactic acid bacteria isolated from chicken gut

Abstract

Abstract Probiotics are live microorganisms or cultures that can benefit host health, maintain and enhance the production and growth of animals, and prevent and control enteric pathogens when given in sufficient doses. They are also one of the alternatives to antibiotic growth promoters in poultry. The current study was to isolate, characterized and evaluate lactic acid bacteria from chicken gut towards use as potential probiotics. Lactic acid bacteria were isolated from broilers and free range chickens, characterized and evaluated for their probiotic properties including antimicrobial activity against pathogens (Salmonella Typhimurium ATCC 14028, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Listeria monocytogenes (clinical isolate) and Enterococus faecalis (clinical isolate), survivability in different acidic environment, tolerance to bile salts, adhesion to chicken intestinal cell, temperature and sodium chloride tolerance. Potential lactic acid bacteria isolated from chickens with outstanding probiotic ability were identified by MALD-TOF mass spectrometer as Lactobacillus reuteri DSM 20016T (B3I13), Pediococcus pentosaccus DSM 20206(B12CA), Lactobacillus reuteri DSM 20016T(B6I3), Lactobacillus reuteri DSM 20016T(F6I8), Lactobacillus reuteri DSM 20016T (B7I05) and Pediococcus pentosaccus DSM 20206(B1I8). The selected strains inhibited all the five pathogens tested with inhibition zone ranging between 11 ± 1 to 20.33 ± 3.055mm, survived in acidic pH and 0.3% bile salt with survival rates of 94.00 to 100.00% and above (100.00%), respectively. The selected lactic acid bacteria were found to have probiotic properties that can be useful in chickens as a supplement for enteric pathogen control and performance improvement.