Three-nucleate Rhizoctonia AG-E - a new subgroup within binucleate Rhizoctonia spp.-Reference-Cited by-同舟云学术

Three-nucleate Rhizoctonia AG-E - a new subgroup within binucleate Rhizoctonia spp.

Published:2023-06-01 Issue: Volume: Page:
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Author:

Moliszewska Ewa¹,Maculewicz Dagna²,Stępniewska Hanna³

Affiliation:

1. University of Opole, Faculty of Natural Sciences and Technology, Institute of Environmental Engineering and Biotechnology, Opole

2. University of Opole, Faculty of Health Sciences, Institute of Health Sciences, Opole

3. University of Agriculture in Krakow, Faculty of Forestry, Department of Forest Ecosystems Protection, Kraków

Abstract

Abstract The article presents a brief review of binucleate Rhizoctonia (BNR) within the Rhizoctonia genus. The genus Rhizoctonia has been classified into two main groups according to the number of nuclei. Binucleate Rhizoctonia strains have two nuclei in each cell, whereas multinucleate Rhizoctonia fungi were observed to have a variable number of nuclei ranging from 4 to 16 in each cell. The study was carried out on 12 BNR isolates originating in southern Poland. Comparison of the ITS1-5,8S-ITS2 sequences of the rDNA of tested isolates with sequences deposited in the GenBank revealed their affiliation to AG-E. Their affiliation to AG was confirmed by anastomosis reactions with tester isolates. The number of nuclei was counted with DAPI staining under a fluorescent microscope, and the diameter of hyphae was also measured. Not all AG-E isolates had the same number of nuclei in their cells: one group among these fungi produced cells with a diverse number of nuclei, usually 3; however, this number ranged from 2 to 4, making the average number of nuclei close to 3. It can be assumed that all isolates with three nuclei belong to this group, which may greatly facilitate the preliminary identification of binucleate isolates of Rhizoctonia spp. belonging to AG-E. This feature may also be a prerequisite for the separation of a new subgroup within AG-E, a subgroup of trinucleate strains (AG-E-3n). The thiamine requirement is not helpful in classifying and describing the AG-E strains.

Publisher

Research Square Platform LLC

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