Wolbachia wMel-mediated effects on dengue virus vertical transmission from Aedes aegypti to their offspring-Reference-Cited by-同舟云学术

Wolbachia wMel-mediated effects on dengue virus vertical transmission from Aedes aegypti to their offspring

Published:2023-05-17 Issue: Volume: Page:
ISSN:
Container-title:
language:
Short-container-title:

Author:

Hue Kien Duong Thi¹,Goncalves Daniela Silva¹,Vi Tran Thuy¹,Long Vo Thi¹,Dui Le Thi¹,Nhu Vu Thi Tuyet¹,Giang Nguyen Thi¹,Trang Huynh Thi Xuan¹,Nguyet Nguyen Minh¹,Phong Nguyen Thanh²,Yacoub Sophie¹,Simmons Cameron P³

Affiliation:

1. Oxford University Clinical Research Unit, Wellcome Trust Africa Asia Programme

2. Hospital for Tropical Diseases, District 5, Ho Chi Minh City

3. Institute for Vector Borne Disease, Monash University, Clayton, Melbourne, VIC 3168

Abstract

Abstract Background: Dengue viruses (DENV) can be transmitted vertically in Aedes aegpti (Ae. aegypti) mosquitoes. Whether the endosymbiont Wolbachia (wMel strain) can reduce the incidence of vertical transmission of DENV from infected females to their offspring is not well understood.Methods We used intrathoracic injection (IT) of DENV-1 to estimate the rate of vertical transmission of the virus in a laboratory colony of Vietnamese Ae. aegypti, both with and without wMel infection. We applied the Maximum Likelihood Estimator (MLE) to calculate the infection rate of individual mosquitoes in their offspring.Results In 6,047 F1 Vietnamese wild-type Ae. aegypti, the MLE of DENV-1 infection was 1.49% (95%CI = 0.73–2.74). In 5,500 wMel infected Ae. aegypti, the MLE was 0%.Conclusions The results reinforce that vertical transmission is a rare event in wild-type mosquitoes and that wMel infection reduces the probability of vertical transmission even further.

Publisher

Research Square Platform LLC

Reference39 articles.

1. Kraemer, M.U., et al. The global distribution of the arbovirus vectors Aedes aegypti and Ae. albopictus. Elife, 2015. 4: p. e08347.

2. The global burden of dengue: an analysis from the Global Burden of Disease Study 2013;Stanaway JD;Lancet Infect Dis,2016

3. A global compendium of human dengue virus occurrence;Messina JP;Sci Data,2014

4. How important is vertical transmission in mosquitoes for the persistence of dengue? Insights from a mathematical model;Adams B;Epidemics,2010

5. First isolation of Aedes flavivirus in the Western Hemisphere and evidence of vertical transmission in the mosquito Aedes (Stegomyia) albopictus (Diptera: Culicidae);Haddow AD;Virology,2013