Stress and recovery of American bullfrog after biometry management: biochemistry and erythrogram responses

Abstract

Abstract The aim was to evaluate the recovery of bullfrog homeostasis after biometry, a routine management. An experiment in a completely randomized design was conducted with six treatments and 10 repetitions: the bullfrog being the experimental unit. Five treatments consisted of evaluating the bullfrog recovery times after biometry (0 - immediately after biometry, 6, 12, 24 and 48 h) and a control (animals in homeostasis in the pen before biometry). Sixty bullfrogs (285.33 ± 10.00 g) were subjected to a 12-h fasting. Subsequently, 50 animals underwent biometry and 10 were used for blood collection before biometry (control). A significant increase was observed in all variables analyzed for animals subjected to density stress, except for total proteins, globulin, and hemoglobin. There was a significant increase in blood glucose and erythrocyte numbers in the subjects immediately after biometry. Total proteins and globulin had no significant difference in any of the groups that underwent biometry. Lactate, albumin, and triglycerides levels were significantly elevated in animals shortly after biometry and remained elevated until 12 h after management. The results showed that 24 h after biometry stress, all the analyzed variables were already at similar levels as to the levels of animals in homeostasis.