Affiliation:
1. University of Nairobi
2. Kenya Medical Research Institute
3. Kenyatta National hospital
Abstract
Abstract
Background
N-acetyl transferase (NAT2) affects metabolism and disposition of isoniazid. Polymorphisms of NAT2 may affect pharmacokinetics of isoniazid (INH) by decreasing concentration of active drug in fast acetylators. High concentrations of drug in slow acetylators may lead to hepatotoxicity; this affects clinical outcomes of patients on therapy.
Objectives
The main objective was to characterize the distribution of selected single nucleotide polymorphisms (SNPs) of NAT2 in patients on IPT at the Comprehensive Care Centre (CCC) of Kenyatta National Hospital (KNH) and to investigate for a relationship between acetylator status and isoniazid induced hepatotoxicity (as indicated by elevated ALT levels).
Methods
The study design was a cross sectional study and entailed collection of patient data. The QIAamp ® DNA Mini kit was used for extraction of DNA and purification of Genomic DNA, followed by DNA sequencing. Data analysis was conducted using SPSS version 25(IBM USA).
Results
The prevalence of the homozygous NAT2 genotype was 19% and that of the heterozygous genotype was 50%. The proportion of the population with slow acetylator alleles was 56% and the proportion of fast acetylator alleles was 44%. Fisher's exact test showed no significant association between ALT levels and NAT2 genotype (P = 0.330).
Conclusion
The study found no significant association between NAT2 genotypes and ALT levels (P = 0.33)
Publisher
Research Square Platform LLC