Vitamin D has therapeutic effects on obesity and hyperandrogenemia in PCOS mouse model induced by low dose DHEA and high-fat diet

Abstract

Abstract Polycystic ovary syndrome (PCOS) is the most complex and common reproductive endocrine disease among reproductive age women. This study aimed to investigate the effects of vitamin D (Vit.D) in PCOS mouse model. Prepubertal female mice were divided into 4 groups randomly: control, PCOS, PCOS with low dose Vit.D(LDVD), and PCOS with high dose Vit.D༈HDVD༉ groups(n = 10 per group). PCOS model was induced by high-fat diet, and subcutaneous injection with 6mg/kg/day dehydroepiandrosterone. After 30 days, 1,25(OH)2D3 was intend to be administered by intraperitoneal injection for 40 consecutive days, 1.3µg/kg/week in LDVD group, and 13µg/kg /week in HDVD group. However, all mice in HDVD group died within two weeks following the Vit.D injections. The body weight of mice and the testosterone levels in PCOS group were significantly higher than those in the control and LDVD groups (P < 0.001). The total cholesterol levels in the control group were lower than those in PCOS and LDVD groups (P < 0.001). Further, the ratio of liver to body weight was different among groups (P < 0.001). Our data illustrates that Vit.D has therapeutic effects on obesity and hyperandrogenemia in PCOS, which may occur through regulation of liver metabolism. However, over dose of Vit.D is poisonous.