Affiliation:
1. Modern Research Center for Traditional Chinese Medicine of Shanxi University
Abstract
Abstract
Astragali Radix (AR) is widely used because of its dual use in medicine and food, and its quality evaluation is of great importance. In this study, a pseudo-targeted metabolomics approach based on scheduled multiple reaction monitoring (sMRM) was developed, and a total of 114 compounds with good linearity, sensitivity and reproducibility were selected for relative quantification. With the help of multivariate and univariate analysis, 26 differential compounds between wild/semi-wild AR (AR-W) and cultivated AR (AR-C) were determined. Then 5 marker compounds were screened out by lasso regression, and further verified by systematic clustering, random forest, support vector machine, and receiver operating characteristic (ROC) analysis. In addition, malonyl-substituted flavonoids showed relative higher content in AR-W. Thus, the malonyl-substitution was the characteristic for flavonoids in AR-W. In conclusion, the application of pseudo-targeted metabolomics and various statistical methods could offer multi-dimensional information for the holistic quality evaluation of AR.
Publisher
Research Square Platform LLC
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