Affiliation:
1. Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya
Abstract
Abstract
Powdery mildew is one of the serious diseases of garden pea which causes a large number of yield losses. Genetic resistance is quite effective, being cost-effective and environment friendly than fungicide applications. In the present studies an initial attempt has been made to identify resistant genotypes against powdery mildew disease developed from hybridization followed by validation of the disease. The newly isolated 44 lines along with four checks were screened under natural and artificial conditions. Ten lines viz., SP7, SN-1, SN-6-1, SN-7-1, SN-2, SN-5-2, SN-6-2, SN-21 and SP-28-1 showed resistant reaction along with check Palam Sumool while 27 lines were identified as moderately resistant in comparison to susceptible check Azad P-1. Besides, six lines namely, SP-2, SP-5, SP-10, SP-24, SA-4 and SP-12-1 gave moderately susceptible reaction along with checks Pb-89 and Palam Priya. Only, SP-19 was categorized as susceptible. The high yielding lines SP-3, SP-6 and SP-22 showed moderately resistant reaction in both natural and artificial conditions. Validation of resistance using molecular markers revealed that neither the parental genotypes nor the progenies possess the er1 gene of JI1559. The er2 linked marker ScOPX-171700 was polymorphic between Palam Sumool and Palam Priya but the marker didn’t show polymorphism between er2 harboring line (JI2480). These results suggested that the lines showing resistance under field conditions may have some other genes or alleles for resistance and further confirmation is needed by developing mapping populations with specific gene or gene combinations.
Publisher
Research Square Platform LLC
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