Construction of eukaryotic expression vector of Apoptin-etag and TAT-Apoptin-etag and their effects on human esophageal squamous cell carcinoma in vitro

Abstract

Abstract Esophageal carcinoma is one of the six most common carcinomas in the world, known for its late clinical presentation, rapid progression and poor survival. Compared with developed countries, the prevalence of esophageal cancer has significant differences in pathology, with significantly higher mortality and regional incidence. Esophageal squamous cell carcinoma (ESCC) is the most common type in China and in recent years, the incidence has shown an upward trend in the county. Considering the characteristics of esophageal cancer in China, seeking new treatments for esophageal squamous cell carcinoma is of great concern to clinicians. Apoptin, which is derived from chicken anemia, and has tumor-specific killing effects, is composed of 121 amino acids. Although the mechanism is not yet explained, it has been extensively studied and used in the treatment of malignant tumors. Here, we selected two human squamous cell carcinoma lines and constructed eukaryotic expression vectors of apoptin and TAT-apoptin fusion protein to investigate their effect on cell proliferation and cell cycle in transfection cells.